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Intermembrane linkage mediated by tubulin.

P Vassilev, M Kanazirska, H T Tien

    Biochemical and Biophysical Research Communications
    |January 16, 1985
    PubMed
    Summary
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    Brain lipid membranes showed interconnected electrical potentials when polymerized tubulin was present. This suggests microtubule networks may link cellular membranes, influencing their electrical activity.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • Cellular membranes maintain electrical potentials crucial for function.
    • Tubulin polymerizes to form microtubules, essential components of the cytoskeleton.
    • Interactions between cellular components can influence electrical signaling.

    Purpose of the Study:

    • To investigate potential electrical communication between lipid membranes mediated by brain tubulin.
    • To determine if polymerized tubulin influences the electrical properties of separate membranes.

    Main Methods:

    • Formation of two brain lipid membranes in the presence of brain tubulin.
    • Simultaneous measurement of electrical potentials across both membranes.
    • Application of electrical pulses to one membrane and observation of effects on the other.

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    Main Results:

    • Electrical pulses applied to one membrane caused potential shifts in the second, non-contacting membrane.
    • This effect was dependent on the presence of polymerized tubulin.
    • No such effect was observed with depolymerized tubulin or in control experiments.

    Conclusions:

    • Polymerized tubulin, forming microtubule networks, appears to facilitate electrical coupling between membranes.
    • Microtubule networks may act as an intercellular communication system, linking membranes or compartments.
    • This suggests a novel role for microtubules in regulating cellular electrical activity.