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Updated: Jul 11, 2026
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
Development of a visual immunosensing platform based on fluorescent microspheres for the rapid detection of
Xiaofang Li1, Maozhong Sun1, Liguang Xu1
1International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, PR China.
Abstract:
Ochratoxins (OTs, including OTA, OTB, and OTC), exhibit nephrotoxicity, carcinogenicity, teratogenicity, and immunotoxicity, contaminating wheat, maize, and rice, so endangering human health. In this study, we designed and screened haptens using computational chemistry to prepare mAb 4G11, which simultaneously recognized OTA, OTB, and OTC with high sensitivity and specificity. We developed a fluorescent microsphere immunochromatographic assay (FMIA), which could detect the total amount of OTs with a visual detection limit of 0.5 ng/mL and a quantification limit of 0.27 ng/mL. Recovery experiments ranged from 95.94 % to 106.01 % with coefficients of variation ranging from 2.74 % to 6.30 %. The results obtained from the FMIA were found to be in alignment with those obtained from liquid chromatography-tandem mass spectrometry. The FMIA reliability was further demonstrated using real samples. FMIA can serve as a valuable instrument for the surveillance of the content of OTs in cereals.
