Effects of compound immobilized bacteria on remediation and bacterial community of PAHs-contaminated soil

Immobilized microorganism technology is expected to enhance microbial activity and stability and is considered an effective technique for removing soil polycyclic aromatic hydrocarbons (PAHs). However, there are limited high-efficiency and stable bacterial preparations available. In this study, alkali-modified biochar (Na@CBC700) was used as the adsorption carrier, sodium alginate (SA) and polyvinyl alcohol (PVA) as embedding agents, and CaCl₂ as the cross-linking agent to prepare immobilized Acinetobacter (CoIMB) through a composite immobilization method. The CoIMB preparation was optimized using response surface methodology and applied to PAH-contaminated soil remediation. Results indicated that CoIMB exhibited improved mechanical strength and microbial activity, achieving degradation rates of 2-5 rings PAHs up to 82.41 %, averaging 1.5 times higher than CK. High dose CoIMB treatment enhanced soil microbial community diversity, enriching Acinetobacter, and increased the abundance of functional genes related to fatty acid metabolism and energy metabolism (K00249, K01897, K00059). This composite immobilized bacterial particle provides a novel, broad-spectrum, and cost-effective solution for remediating organic pollutants in soil environments.