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Updated: Jul 12, 2026

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
Published on: September 8, 2011
Time-dependent changes in the binding parameters of muscarinic cholinergic receptors in the rat brain
Abstract:
Using quantitative receptor autoradiography, effects of several incubation times on the binding parameters for [3H]quinuclidinyl benzilate [(3H]QNB) binding were investigated in 5 discrete regions of the rat brain. There were no differences in Bmax-values between 3 incubation times. On the other hand, Kd(app)-values markedly depended on the duration of incubation time. Furthermore, Scatchard plots at low [3H]QNB concentrations showed an abnormal binding behavior, i.e. deviation from the straight line representing a single population of the binding site.
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