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Related Experiment Video

Updated: Jun 4, 2025

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

The Epileptiogenic Modified Therapy: Regulating the Dynamic of Microglia via ROS-Responsive Cascade Nano-Formulation.

Jiaxin Li¹, Shuai Liu², Chenghan Chen^1,3

¹Department of Neurosurgery, The National Key Clinical Specialty, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, The Neurosurgery Institute of Guangdong Province, Zhujiang Hospital Southern Medical University, Guangzhou, 510280, China.

Advanced Healthcare Materials

|December 23, 2024

Summary

This summary is machine-generated.

A novel nano-formulation effectively transforms reactive oxygen species into nitric oxide, reducing seizure severity and altering microglial dynamics in epilepsy models. This approach offers a new strategy for managing epilepsy by targeting neuroinflammation.

Keywords:

dynamic epilepsy microglia nano‐formulation nitric oxide

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Lipidomics and Transcriptomics in Neurological Diseases

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Area of Science:

Neuroscience
Pharmacology
Biomaterials

Background:

Epilepsy treatment faces challenges with antiseizure medicines (ASMs) failing to modify disease progression.
Reactive oxygen species (ROS) and nitric oxide (NO) play roles in neuroprotection and seizure tolerance.
Microglia, brain immune cells, are implicated in epilepsy initiation and progression.

Purpose of the Study:

To develop a ROS-responsive cascade nano-formulation (RRCN) that converts ROS into NO.
To investigate the therapeutic potential of RRCN in reducing seizure severity and modifying microglial activity.
To elucidate the NO/HIF/ErBb2 pathway's role in RRCN's anti-epileptic effects.

Main Methods:

Development of a ROS-responsive cascade nano-formulation (RRCN) designed to transform ROS into NO.
Utilizing the kainic acid (KA) epilepsy model to assess RRCN's impact on seizure parameters.
Analyzing microglial morphology and neuron-microglia interactions in the hippocampus using microscopy and pathway analysis.

Main Results:

RRCN significantly reduced seizure severity, prolonged seizure latency, and extended inter-seizure intervals.
RRCN did not increase the latency of generalized seizures.
RRCN reversed aberrant microglial morphology and neuron-microglia interactions in the hippocampus, mediated by the NO/HIF/ErBb2 pathway.

Conclusions:

The developed RRCN effectively leverages ROS to generate NO, offering a novel therapeutic strategy for epilepsy.
RRCN demonstrates significant efficacy in mitigating seizure activity and neuroinflammation.
RRCN inhibits seizure generalization by modulating microglial dynamics via the NO/HIF/ErBb2 pathway.