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Color-Coded Traffic Signal Method Combined with Nanodiamond Quantum Sensing for Accurate miRNA Detection.

Wei Wang1,2, Youqiang Xing1,2, Lei Liu1,2

  • 1School of Mechanical Engineering, Southeast University, Nanjing 211189, People's Republic of China.

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This study developed a novel method using fluorescent nanodiamonds (FNDs) to reduce background noise for early tumor biomarker detection. The technique achieves highly sensitive, single-molecule detection of cancer-related microRNAs.

Keywords:
Accurate detectioncolor-codedmiRNAsnitrogen-vacancy centersquantum sensingtraffic signal method

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Area of Science:

  • Biomedical Engineering
  • Quantum Sensing
  • Nanotechnology

Background:

  • Background noise hinders accurate detection of early tumor biomarkers.
  • Developing sensitive and specific detection methods is crucial for early cancer diagnosis.

Purpose of the Study:

  • To introduce a novel method for reducing background noise to enhance early tumor biomarker detection accuracy.
  • To utilize fluorescent nanodiamonds (FNDs) with nitrogen-vacancy centers for improved biomarker signal-to-noise ratio.

Main Methods:

  • Combined a color-coded signaling approach with FNDs' fluorescent and quantum spin properties.
  • Employed optical detection magnetic resonance (ODMR) for distinguishing FND signals from background noise.
  • Utilized red, green, and yellow signals to indicate analyte presence, absence, or need for further analysis.

Main Results:

  • Achieved detection of breast cancer-related miRNA-21 and miRNA-96 at concentrations as low as 1 femtomolar (fM).
  • Demonstrated single-molecule detection capability within a 100 × 100 μm² area.
  • Successfully distinguished FND signals from background fluorescence.

Conclusions:

  • The developed method effectively reduces background noise for enhanced biomarker detection.
  • This technique offers high sensitivity and accuracy for identifying cancer-related microRNAs.
  • The approach is suitable for biomarker detection in challenging high-background fluorescence environments.