Integrated Near-Infrared Light-Driven Janus Micromotor-Fluorescent Nanodiamond Dynamic Sensing Platform for Active...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Background:

• Efficient detection of microRNAs and proteins is crucial for early cancer diagnosis.
• Conventional fluorescence assays face limitations like photobleaching and slow mass transport, hindering signal stability.

Purpose of the Study:

• To develop a near-infrared (NIR)-driven dynamic biosensing platform (JMMs-FNDs) for enhanced biodetection.
• To overcome limitations of static incubation methods in fluorescence assays.

Main Methods:

• Integration of fluorescent nanodiamonds (FNDs) with self-propelled H-MnO2-Au Janus magnetic micromotors (JMMs).
• Utilizing NIR irradiation to induce thermophoretic propulsion for enhanced mass transport and target molecule enrichment.
• Employing a mesoporous hollow MnO2 shell for improved target-receptor interactions.

Main Results:

• Achieved a significant increase in capture efficiency for miRNA-21 from 52.48% to 78.46%.
• Demonstrated ultrasensitive detection with a limit of 0.72 fM, a 40-fold improvement over static methods.
• Successfully performed quantitative detection of MUC1 using a strand-displacement recognition strategy.

Conclusions:

• The JMMs-FNDs platform integrates self-propulsion with stable fluorescence signaling for efficient and highly sensitive fluorescence biodetection.
• This dynamic approach enhances capture efficiency and reduces detection limits for cancer biomarkers.