Benchtop centrifugation: An effective method for reducing lipaemia associated interference in grossly lipaemic...

Area of Science:

• Clinical Chemistry
• Laboratory Medicine
• Analytical Biochemistry

Background:

• Grossly lipaemic samples cause significant analytical errors, impacting patient care.
• Traditional ultracentrifugation for lipaemia reduction is limited by accessibility and cost.
• Benchtop centrifugation is explored as a practical alternative.

Purpose of the Study:

• To evaluate the efficacy of benchtop centrifugation in reducing lipaemia in serum samples.
• To assess the impact of benchtop centrifugation on the accuracy of core analyte measurements.

Main Methods:

• Fifty-three lipaemic serum samples were centrifuged at 18840 g for varying durations.
• Lipaemia levels were measured before and after centrifugation.
• Core analytes were measured on samples before and after 30 minutes of centrifugation.

Main Results:

• 90% of samples achieved ideal or adequate lipaemia levels after 5 minutes of centrifugation.
• All samples were suitable for analysis after 30 minutes of centrifugation.
• No clinically significant changes in analyte concentrations were observed post-centrifugation.

Conclusions:

• Benchtop centrifugation is a promising method for reducing lipaemia while maintaining reliable results.
• Further research with larger sample sizes and temperature analysis is needed.
• This technique offers potential laboratory utility balancing practicality and accuracy.