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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

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Published on: March 8, 2024

Human colon organoid differentiation from induced pluripotent stem cells using an improved method.

I-Ting Lee¹, Yu Takahashi¹, Takashi Sasaki¹

¹Food Biochemistry Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

|December 23, 2024

Summary

This study refines human colon organoid (HCO) differentiation from induced pluripotent stem cells (iPSCs). Optimized protocols enhance colon-specific markers and maturation for better disease modeling.

Keywords:

BMP2 signaling HOXD13 SATB2 colon organoids induced pluripotent stem cells

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Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

Area of Science:

Stem cell biology
Gastrointestinal physiology
Organoid technology

Background:

Colonic epithelium is vital for gastrointestinal homeostasis.
Human colon organoids (HCOs) are valuable models for studying colonic physiology.
Current methods for differentiating induced pluripotent stem cells (iPSCs) into HCOs lack standardization, limiting their use.

Purpose of the Study:

To optimize the differentiation protocol for generating mature human colon organoids (HCOs) from iPSCs.
To compare existing protocols and identify key factors for enhanced colonic identity and maturation.
To improve the reliability of HCOs as models for colonic research.

Main Methods:

Comparative analysis of two established iPSC to HCO differentiation protocols.
Utilized transient bone morphogenetic protein 2 (BMP2) signaling activation.
Employed adenovirus-mediated transduction of HOXD13 or SATB2 transcription factors.

Main Results:

The protocol involving transient BMP2 activation showed superior upregulation of colon-specific markers.
Co-administration of BMP2 with HOXD13 or SATB2 transduction significantly enhanced colonic identity.
The optimized protocol resulted in more mature HCOs.

Conclusions:

Refined differentiation protocols significantly improve the generation of mature human colon organoids.
Enhanced HCOs provide a more robust model for investigating colonic epithelial biology and pathology.
This advancement facilitates further research into gastrointestinal diseases.