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Seawater Sampling and Collection

Seawater Sampling and Collection

Published on: June 17, 2009

Novel Drop-Sampler for Simultaneous Collection of Stereo-Video, Environmental DNA and Oceanographic Data.

Samuel Thompson^1,2,3, Simon Jarman^1,2,3, Kingsley Griffin^1,2

¹School of Biological Sciences The University of Western Australia Perth WA Australia.

Ecology and Evolution

|December 24, 2024

Summary

Environmental DNA (eDNA) and visual surveys enhance marine biota assessments. Combining both methods reveals complementary data, improving species diversity estimates and understanding sampling biases.

Keywords:

BRUVs biodiversity drop‐camera eDNA fish ecology metabarcoding sampling method stereo‐video

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Last Updated: Jul 6, 2026

Seawater Sampling and Collection

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Published on: June 17, 2009

Development of New Methods for Quantifying Fish Density Using Underwater Stereo-video Tools

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Methods for Image-based Surveys of Benthic Macroinvertebrates and Their Habitat Exemplified by the Drop Camera Survey for the Atlantic Sea Scallop

Methods for Image-based Surveys of Benthic Macroinvertebrates and Their Habitat Exemplified by the Drop Camera Survey for the Atlantic Sea Scallop

Published on: July 2, 2018

Area of Science:

Marine ecology
Molecular ecology
Biodiversity assessment

Background:

Environmental DNA (eDNA) is a growing tool for marine surveys.
Ground-truthing eDNA data with visual surveys is crucial for understanding method biases.
Traditional methods need enhancement for comprehensive biodiversity monitoring.

Purpose of the Study:

To develop and test a robust, rapidly deployable system for simultaneous eDNA and stereo-video data collection.
To compare fish assemblages inferred from eDNA and visual surveys.
To assess the complementarity and biases of eDNA and visual sampling methods.

Main Methods:

Designed a system for rapid, simultaneous collection of water samples for eDNA and stereo-video imagery.
Deployed the system on the seafloor for data collection in various habitats and depths (up to 600m).
Compared eDNA-inferred fish assemblages with data from baited remote underwater video (stereo-BRUV) samples.

Main Results:

Combining eDNA and visual survey data increased fish assemblage diversity by approximately 6.5% compared to eDNA alone.
Significant differences were found in the fish assemblage composition between the two methods.
The study highlighted the inherent biases and complementarity of each sampling technique.

Conclusions:

The integrated approach provides a more comprehensive understanding of marine fish assemblages.
Future advancements in eDNA metabarcoding should aim to differentiate abundance and life stages.
Further research is needed to correlate eDNA data with imagery-derived biological and habitat information.