Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 10, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Development of a web-based high-throughput marker design program: CAPS (cleaved amplified polymorphic sequence)

Joo-Seok Park^1,2, Yoram Choi¹, Jin-Hyun Kim³

¹Department of Applied Bioscience, Dong-A University, Saha-Gu Nakdong-Daero 550 beongil 37, Busan, 49315, Republic of Korea.

|December 24, 2024

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AI-Guided DNA-Free and Genotype-Independent Genome Editing for Soybean Improvement.

Plants (Basel, Switzerland)·2026

Same author

Botulinum neurotoxin for lip flip.

JPRAS open·2026

Same author

Comparative genomics and phenotypes of Listeria monocytogenes isolated from enoki mushrooms in South Korea and China.

Food microbiology·2026

Same author

NMR-Based Metabolic Profiling of Biobank Derived Blood Samples for the Identification of Liver Disease Biomarkers.

Biomolecules & therapeutics·2026

Same author

Bayesian colocalization of psychiatric disorder GWAS signals with molecular quantitative trait loci highlights histone involvement and neuroimmune dysregulation in pathogenesis.

Psychiatry research·2026

Same author

Asymmetrical Correction of Facial Rotation Using Unilateral Thread Insertion.

Aesthetic plastic surgery·2026

A new digital platform, CAPS Maker, streamlines the design of DNA markers for soybean breeding. This tool facilitates the development of improved soybean varieties with desirable traits by efficiently handling large genomic datasets.

Area of Science:

Plant genetics
Bioinformatics
Crop science

Background:

Genetic markers are essential for developing crops with improved agronomic traits.
Next-generation sequencing (NGS) and bioinformatics tools accelerate marker development.
Existing tools are limited to short sequences, hindering genome-wide marker design in large datasets.

Purpose of the Study:

To develop an automated system for designing genome-wide molecular markers in soybean.
To create a user-friendly platform for efficient marker development using big data.

Main Methods:

Development of the CAPS Maker digital platform with a graphical interface.
Integration of a 'SNP Browser' and 'Primer Table' for systematic marker design.
Inclusion of internal programs, such as the eHT-PCR module, for primer pair design in complex genomes.

Keywords:

CAPS Maker Genetic marker Re-sequencing SNP Soybean Variant

More Related Videos

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 10, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Main Results:

The CAPS Maker platform was developed for designing cleaved amplified polymorphic sequence (CAPS) and derived CAPS (dCAPS) markers in soybeans.
The platform features user-friendly tools for marker design and primer pair selection.
Internal programs were incorporated to handle highly duplicated genomes.

Conclusions:

The CAPS Maker platform's effectiveness and accuracy were validated experimentally.
The platform enables breeders to easily design CAPS/dCAPS markers for developing new soybean cultivars.
The CAPS Maker is accessible online for public use.