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Updated: May 8, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
Published on: July 25, 2022
Electronically Controlled Dual-Wavelength Switchable SRS Fiber Amplifier in the NIR-II Region for Multispectral
Hwidon Lee1, Markus R Seeger2, Brett E Bouma3
1Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea; Engineering ResearchCenter forColor-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea; Harvard Medical School, Boston, MA 02114, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.
Abstract:
Photoacoustic microscopy (PAM) is a high-resolution and non-invasive imaging modality that provides optical absorption contrast. By employing dual- or multiple-wavelength excitation, PAM extends its capabilities to offer valuable spectroscopic information. To achieve efficient multispectral PAM imaging, an essential requirement is a light source characterized by a high repetition rate and switching rate, a ≈microjoule pulse energy, and a ≈nanosecond pulse duration. However, there exists a notable deficiency in suitable light sources, particularly in the near-infrared-II window. In this study, a custom-built all-fiber-based light source is reported that provides >3 μJ, 2 ns pulses with a repetition rate of 200 kHz. Digitally addressed semiconductor seed lasers, followed by stimulated Raman scattering amplification, enabled arbitrary sequences of pulses having wavelengths of either 1168.4 or 1202.1 nm. In a switching mode of operation, a 100 kHz switching rate is used to alternate between these wavelengths in even/odd pulses. Furthermore, a high-resolution multispectral photoacoustic microscopy of three polymer samples is demonstrated with the proposed light source.
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