Related Experiment Video
Updated: Jun 4, 2025

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
Published on: May 28, 2015
Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)
Aistė Petruškevičiūtė1, Ugnė Šimuliūnaitė2, Catalina M Polanco1
1VU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, Lithuania.
Abstract:
Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization. Here we created knock-in reporter human iPS cell lines carrying the MARINA reporter using SpCas9 programmable nuclease and characterize a heterozygous clone.
Related Concept Videos
Induced Pluripotent Stem Cells
Somatic...
Somatic to iPS Cell Reprogramming

