Depth weakens effects of long-term fertilization on dissolved organic matter chemodiversity in paddy soils

Dissolved organic matter (DOM) is pivotal for soil biogeochemical processes, soil fertility, and ecosystem stability. While numerous studies have investigated the impact of fertilization practices on DOM content along soil profiles, variations in DOM chemodiversity and the underlying factors across soil profiles under long-term fertilization regimes remain unclear. Using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and high-throughput sequencing, this study investigated DOM composition characteristics and microbial community compositions across different soil layers (0-20, 20-40, 40-60, and 60-100 cm) in paddy soil under different long-term fertilization treatments, including Control (no fertilizer), NPK (mineral NPK fertilizer), NPKHS (NPK fertilizer with half straw return), and NPKS (NPK fertilizer with full straw return). The results revealed that fertilization regimes significantly increased soil TC, TN, and NO₃^- contents, as well as DOM chemodiversity in the top soil layer, particularly under NPKHS and NPKS treatments. Both the DOM chemodiversity and bacterial diversity decreased with soil depth. However, below 0-20 cm, DOM chemodiversity was not significantly affected by fertilization treatments. Co-occurrence network analysis further showed that microbial decomposition primarily drove the changes in DOM composition across soil profile. Overall, our study suggests that long-term NPK fertilization and straw return significantly increased DOM chemodiversity only in the top layer of paddy soil by regulating soil TC, TN, and NO₃^- contents. Our study provides useful information regarding the vertical molecular composition of DOM and enhances the understanding of DOM chemodiversity along soil profile in rice paddy ecosystems.