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Related Experiment Video

Updated: May 8, 2025

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex
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Extracellular Optogenetics to Interrogate Unmodified Primary Cell Functions.

Morgane Jaeger1, Renaud Vincentelli2, Rémi Lasserre3

  • 1Aix Marseille Université, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, CIML, Turing Center for Living Systems, Marseille, France.

Methods in Molecular Biology (Clifton, N.J.)
|December 26, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed an extracellular optogenetic system for controlling T cell receptor (TCR) activation. This method bypasses genetic modification, enabling study of primary T cells in their natural state.

Keywords:
Extracellular optogeneticsNonengineered primary cellPhytochromeReceptor activationRed/far-red optogeneticsT cell receptorT cells

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Area of Science:

  • Immunology
  • Molecular Biology
  • Biotechnology

Background:

  • Optogenetics typically requires genetic engineering of cells, hindering research on primary cells.
  • Studying primary cells in their native state is crucial for understanding biological processes.
  • Murine naïve T cells are genetically challenging to manipulate, limiting optogenetic applications.

Purpose of the Study:

  • To present a novel, fully extracellular optogenetic system for controlling T cell receptor (TCR) activation.
  • To enable the study of primary T cells, specifically murine naïve T cells, without genetic modification.
  • To demonstrate the versatility of the system for different cell types and species.

Main Methods:

  • Development of a fully extracellular optogenetic system.
  • Application of the system to murine naïve T cells.
  • Demonstration of reversible light control over TCR activation.

Main Results:

  • Successful implementation of an extracellular optogenetic system for T cell activation.
  • Achieved reversible light-induced control of TCR signaling in primary T cells.
  • The system proved effective on a cell model difficult to genetically modify.

Conclusions:

  • The extracellular optogenetic system offers a versatile tool for manipulating primary T cell activation.
  • This approach overcomes limitations of genetic modification for studying cells in their native state.
  • The system has broad applicability for diverse cell systems and species.