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Paper-Based Device for Phenolic Content Determination in Tea Extracts.

Rachma Athaya Silver1, Eka Noviana2, Muhammad Al-Fatih Ash Shiddiq1

  • 1Pharmacy Undergraduate Program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Phytochemical Analysis : PCA
|December 26, 2024
PubMed
Summary
This summary is machine-generated.

A new paper-based analytical device (PAD) offers a simple, rapid method for determining total phenolic content (TPC) in tea extracts. This colorimetric sensor provides accurate results comparable to traditional spectrophotometry.

Keywords:
paper‐based devicephenolic compoundplant extractrapid estimationtotal phenolic content

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biochemistry

Background:

  • Phenolic compounds are vital in natural product-derived medicines, nutraceuticals, and cosmeceuticals.
  • Traditional spectrophotometry for quantifying phenolic compounds is laborious, time-consuming, and reagent-intensive.

Purpose of the Study:

  • To develop a simple, point-of-need colorimetric sensor for rapid determination of total phenolic content (TPC).
  • To create a user-friendly device for analyzing TPC in tea extracts.

Main Methods:

  • Development of a radial paper-based analytical device (PAD).
  • Utilized the Folin-Ciocâlteu colorimetric reaction for TPC detection.
  • PAD enabled both quantitative (image analysis) and semiquantitative (color chart) measurements.

Main Results:

  • Successful application of PAD for TPC determination in tea extracts using various solvents (water, methanol, ethanol) with high accuracy and precision.
  • TPC values obtained from PAD correlated well with visible spectrophotometry.
  • Semiquantitative analysis using a color reference card achieved over 95% accuracy.
  • Optimal storage conditions identified as 4°C in a light-protected, vacuum-sealed container for device stability.

Conclusions:

  • The developed PAD is a promising tool for simple, rapid, and accurate TPC estimation in plant extracts.
  • This sensor offers a practical alternative to conventional methods for phenolic compound analysis.
  • The device facilitates point-of-need analysis, reducing time and reagent consumption.