Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

2.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Second-Generation ELZA-sub400 Protocol: Individualized High-Fluence Cross-Linking for Ultra-Thin Keratoconus Corneas.

American journal of ophthalmology·2026
Same author

Optimizing genomic language models for promoter prediction: a comparative study of tokenization and cross-species learning.

NAR genomics and bioinformatics·2026
Same author

Accelerated CXL Versus Accelerated Contact-Lens Assisted CXL Treatment for Progressive Keratoconus-A 3-Year Retrospective Comparative Follow-Up.

Journal of clinical medicine·2025
Same author

Preventing Shunt Launch: A Novel Technique for Extraocular Flushing of the PreserFlo MicroShunt.

Clinical & experimental ophthalmology·2025
Same author

Exosomes in Ocular Health and Disease.

Current eye research·2025
Same author

Alpha-1 antitrypsin promotes re-epithelialization by regulating inflammation and migration.

Frontiers in immunology·2025
Same journal

Impact of Subretinal Drusenoid Deposits on Ellipsoid Zone-Related Thickness Metrics.

Investigative ophthalmology & visual science·2026
Same journal

Proteomic Profiling of Optic Nerves From SMOX-Deficient Mice Identifies Regulators of Neuroinflammation and Axonal Damage in Optic Neuritis.

Investigative ophthalmology & visual science·2026
Same journal

Aflibercept and Faricimab Equipotently Restore Endothelial Barrier Function.

Investigative ophthalmology & visual science·2026
Same journal

Spatial Decomposition of Longitudinal RNFL Maps Reveals Distinct Modes of Glaucomatous Progression With Structure-Function and Genetic Signatures.

Investigative ophthalmology & visual science·2026
Same journal

The CXXC1-IGFBP6 Axis Maintains Corneal Epithelial Differentiation via H3K4me3-Dependent Transcriptional Activation.

Investigative ophthalmology & visual science·2026
Same journal

Archetypal Visual Field Analysis of Patients With Chronic Leber Hereditary Optic Neuropathy in Relation to Visual Recovery.

Investigative ophthalmology & visual science·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Integrated Profiling of Extracellular Vesicle microRNA Impact on Trabecular Meshwork mRNA Expression: Insights From

Efrat Cohen-Davidi1, Valeria Feinstein2, Boris Knyazer3

  • 1Department of Software and Information Systems Engineering, Faculty of Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Investigative Ophthalmology & Visual Science
|December 27, 2024
PubMed
Summary
This summary is machine-generated.

Extracellular vesicles (EVs) from stressed non-pigmented ciliary epithelial (NPCE) cells alter gene expression in human trabecular meshwork (HTM) cells, revealing potential mechanisms in primary open-angle glaucoma (POAG) and new therapeutic targets.

More Related Videos

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry
07:29

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry

Published on: October 6, 2023

Related Experiment Videos

Last Updated: May 10, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry
07:29

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry

Published on: October 6, 2023

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Molecular Biology

Background:

  • Primary open-angle glaucoma (POAG) is a leading cause of irreversible blindness.
  • The role of extracellular vesicles (EVs) in POAG pathogenesis is an emerging area of research.
  • Oxidative stress in ocular tissues is implicated in glaucoma progression.

Purpose of the Study:

  • To investigate the impact of microRNAs (miRNAs) within EVs from oxidative stress-induced non-pigmented ciliary epithelial (NPCE) cells on human trabecular meshwork (HTM) cell gene expression.
  • To elucidate the molecular mechanisms by which NPCE-derived EVs may contribute to POAG.

Main Methods:

  • NPCE cells were subjected to oxidative stress, and EVs were isolated.
  • HTM cells were treated with EVs from control and stressed NPCE cells.
  • Microarray analysis identified differentially expressed miRNAs in EVs and mRNAs in HTM cells.
  • Bioinformatics analyses explored miRNA-mRNA interactions and associated biological pathways.

Main Results:

  • 54 differentially expressed miRNAs were identified in EVs from stressed NPCE cells.
  • Treatment of HTM cells with stressed NPCE EVs resulted in 88 upregulated and 58 downregulated genes.
  • Upregulated genes were enriched in extracellular matrix organization and cell adhesion pathways, while downregulated genes were linked to oxidative phosphorylation.
  • A significant number of upregulated genes were identified as targets of downregulated miRNAs, forming relevant networks.

Conclusions:

  • NPCE-derived EVs carrying specific miRNAs play a role in modulating HTM cell gene expression under oxidative stress.
  • These findings suggest novel mechanisms contributing to POAG pathogenesis.
  • The identified miRNA-mRNA interactions represent potential therapeutic targets for POAG.