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Updated: May 8, 2025

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights
Stephen C Whisson1, Lydia R J Welsh1, Ramesh R Vetukuri2
1Cell and Molecular Sciences Department, The James Hutton Institute, Invergowrie, Dundee, UK.
Abstract:
RNA silencing is a core cellular process that acts to defend the genome against potentially damaging genetic elements such as viruses and transposons. It has been extensively characterized in many eukaryotes and exploited as a tool for determining gene function through removing the activity of specific genes. It has also been used in Phytophthora species to reveal genes involved in different lifecycle stages. In this chapter, we provide guidelines and outline considerations for carrying out RNA silencing experiments in Phytophthora.
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