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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

Isolating high-quality RNA for RNA-Seq from 10-year-old blood samples.

Charlene Portelli¹, Elisa Seria¹, Ritienne Attard¹

¹Department of Applied Biomedical Science, Faculty of Health Sciences, University of Malta, Msida, 2080, MSD, Malta.

Scientific Reports

|December 27, 2024

Summary

This summary is machine-generated.

High-quality RNA for transcriptomics can be isolated from blood samples stored for 10 years. A modified Zymo Research Quick-RNA kit protocol preserves RNA integrity for RNA sequencing analysis.

Keywords:

Biobanking Long-term storage Multi-omics RNA isolation RNA-Seq Transcriptomics

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Last Updated: May 8, 2025

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Published on: August 6, 2009

Area of Science:

Molecular Biology
Biobanking and Sample Preservation

Background:

RNA analysis, especially RNA Sequencing, is crucial in research and diagnostics.
RNA's instability necessitates robust storage and isolation methods to maintain integrity.
Long-term, cost-effective blood sample storage with minimal volume is desirable for large collections.

Purpose of the Study:

To assess the feasibility of isolating high-quality RNA from long-term stored blood samples.
To evaluate a modified RNA isolation protocol for improved RNA integrity.
To compare RNA quality from long-term stored samples with fresh samples.

Main Methods:

Blood samples (EDTA and citrate) were stored at -85 °C for 10 years in Boom's lysis buffer.
RNA was isolated using a modified Zymo Research Quick-RNA kit protocol.
RNA integrity and quality were assessed, and RNA Sequencing was performed.

Main Results:

High-quality RNA suitable for transcriptomics was successfully isolated from 10-year-old stored blood samples.
The modified Zymo Research protocol significantly improved RNA integrity compared to the original Boom method.
RNA Sequencing yielded high-quality data comparable to studies using recently collected blood.

Conclusions:

Long-term storage of blood in Boom's lysis buffer at -85 °C preserves RNA for high-quality downstream analysis.
A modified Zymo Research Quick-RNA kit protocol offers an effective method for isolating RNA from aged samples.
This approach supports large-scale biobanking and RNA sequencing initiatives.