Optimization of the quality of sea buckthorn juice by enzymatic digestion and inoculation sequence

Sea buckthorn, rich in nutrients and bioactive compounds such as phenolics, fatty acids, and vitamins, presents processing challenges due to its intense sourness and bland flavor. This study addresses key challenges in flavor enhancement and sourness reduction by evaluating the effects of pectinase treatment and inoculation sequences on the overall quality. Optimal malic acid degradation and antioxidant occurred when Schizosaccharomyces pombe (S. pombe) was inoculated after pectinase digestion of the pulp, while sequential inoculation with Saccharomyces cerevisiae and S. pombe produced the most favorable flavor profile. S. pombe effectively promoted the degradation of malic and quinic acids during fermentation, improving color, antioxidant activity, and flavor characteristics. These findings highlight the critical role of pectinase digestion and inoculation sequence, offering practical guidance for optimizing large-scale fermentation processes and strain selection to develop innovative sea buckthorn beverages and enhance their market potential.