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Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda:
Ha Yeun Song1, Young Ji Choi1, Dea-Sung Lee2
1Honam National Institute of Biological Resources, Mokpo, 58762, Republic of Korea.
Objective:
The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing.
Results:
Out of the 63 loci examined, 26 were effectively amplified in a sample of 100 individuals. These 17 loci, comprising four tri-nucleotide and 13 tetra-nucleotide repeats, exhibited a range of 4 to 26 alleles per locus (with an average of 14.1 alleles) across 100 samples from three O. stimpsoni populations. The mean observed and expected heterozygosities were calculated to be 0.885 and 0.836, respectively. These 17 newly identified polymorphic microsatellite loci hold valuable utility for investigating the genetic structure and diversity of this protected marine species.
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