Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Co-occurring ferns orchestrate rhizosphere microbiome assembly driving divergent antimony adaptation at the XKS Mine, China.

Ecotoxicology and environmental safety·2026

Same author

Couple-level dyslipidemia and embryological and cumulative pregnancy outcomes in IVF/ICSI cycles: a single-center retrospective cohort study.

Frontiers in endocrinology·2026

Same author

A Novel Magnetically Targeted Intramedullary (MagIC-TI) Xenograft Model for Precise Leukemia Modeling and Drug Resistance Evaluation in the Bone Marrow Niche.

Journal of immunology research·2026

Same author

Multidimensional effects of personalized exercise intervention in type 2 diabetes: a randomized controlled trial.

Science bulletin·2026

Same author

The mineralocorticoid receptor and its antagonist finerenone regulate hepatic lipid accumulation via the AMPK/SREBP1/FASN signaling.

Molecular and cellular endocrinology·2026

Same author

Sulfate Formation during Heatwaves: Perspective from Observation-Constrained Stabilized Criegee Intermediates.

Environmental science & technology·2026

Search research articles

Related Experiment Video

Updated: May 7, 2025

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Published on: March 28, 2022

Mettl14 and Mettl3 Work Cooperatively to Regulate Retinal Development.

Dan Chen¹, Yanling Xin¹, Jingyi Guo¹

¹State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.

Cell Biochemistry and Function

|December 31, 2024

Summary

The study reveals that METTL14 and METTL3 are essential for proper retinal development. Their combined absence disrupts retinal structure and cell distribution, highlighting their cooperative role in the eye.

Keywords:

Mettl14 Mettl3 N6‐methylenadenosine retinal development

More Related Videos

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Published on: June 28, 2011

Related Experiment Videos

Last Updated: May 7, 2025

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Published on: March 28, 2022

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Published on: June 28, 2011

Area of Science:

Molecular Biology
Developmental Biology
Genetics

Background:

N⁶-methyladenosine (m⁶A) is the most prevalent epitranscriptomic modification in eukaryotic messenger RNAs (mRNAs).
m⁶A modification regulates diverse aspects of mRNA metabolism and function.
The m⁶A methyltransferase complex, including METTL14 and METTL3, plays a key role in its deposition.

Purpose of the Study:

To investigate the roles of METTL14 and METTL3 in retinal development using a conditional knockout system.
To determine the specific functions of these core m⁶A methyltransferase components in the developing retina.

Main Methods:

Utilized the Cre-Loxp conditional knockout system in mice.
Generated double knockout models for Mettl14 and Mettl3 in retinal cells.
Analyzed retinal structure, cell proliferation, cell generation, and cell distribution.

Main Results:

The absence of both Mettl14 and Mettl3 led to significant retinal structural disturbances.
Prolonged proliferation of retinal progenitor cells was observed in the double knockout.
While cell generation was not affected, the distribution of various retinal cell types was severely disrupted.
The combined deletion of Mettl14 and Mettl3 exhibited a more pronounced phenotype compared to the single deletion of Mettl14.

Conclusions:

METTL14 and METTL3 function cooperatively in regulating retinal development.
These core m⁶A methyltransferases are critical for maintaining retinal structure and proper cell organization.