Emerging phospholipid-targeted affinity materials for extracellular vesicle isolation and molecular profiling
Huixia Feng1, Han Gao1, Jian Chen1
1Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; School of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049, China.
Journal of Chromatography. A
|January 1, 2025
Summary
This review highlights advances in phospholipid-targeted affinity strategies for isolating extracellular vesicles (EVs) from complex samples. These methods enable efficient EV separation and molecular detection for improved physiological and pathological studies.
Area of Science:
- Biochemistry and Molecular Biology
- Biotechnology
- Analytical Chemistry
Background:
- Extracellular vesicles (EVs) are crucial in physiological and pathological processes, carrying diverse biomolecules.
- Efficient and selective isolation of EVs from complex biological samples is essential for their study.
- Lipid-based interactions are increasingly leveraged for EV separation and analysis.
Purpose of the Study:
- To review recent progress in lipid membrane-targeted affinity strategies for EV isolation and molecular detection.
- To discuss various affinity ligands and their interactions with EV lipids.
- To summarize the design, construction, and mechanisms of affinity interfaces for EV separation.
Main Methods:
- Focus on phospholipid-targeted affinity materials and platforms.
- Discussion of affinity ligands including lipids, peptides, small molecules, and aptamers.
- Analysis of molecular interactions between ligands and EV lipids.
- Summarization of affinity interface design, construction, and mechanisms of action.
Main Results:
- Recent advances in phospholipid-targeted affinity strategies enable high-performance EV isolation.
- These strategies facilitate EV separation from complex biosamples.
- Downstream proteomic, lipidomic, and metabolic profiling of isolated EVs is improved.
Conclusions:
- Lipid membrane-targeted affinity strategies represent a significant advancement in EV isolation and analysis.
- Further development of next-generation phospholipid-targeted EV separation platforms is promising.
- These platforms hold potential for enhanced understanding and interference of physiological and pathological processes.
