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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Jun 4, 2025

Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

Published on: October 28, 2021

[Advances in tooth development studies based on single-cell RNA sequencing].

N Jiang¹, X T Zhu¹, Y Liu¹

¹Central Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, Beijing100081, China.

Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology

|January 1, 2025

Summary

This summary is machine-generated.

Single-cell RNA sequencing reveals cellular heterogeneity during tooth development. This advanced technology aids understanding of developmental trajectories and identifies targets for prosthodontics and regeneration.

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Last Updated: Jun 4, 2025

Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

Published on: October 28, 2021

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Area of Science:

Developmental Biology
Genomics
Regenerative Medicine

Background:

Tooth development involves complex interactions between ectoderm-derived epithelium and cranial neural crest-derived mesenchyme.
Cellular heterogeneity exists even within the same cell populations during development.
Understanding these intricate processes is crucial for advancements in dental science.

Purpose of the Study:

To summarize recent advances in applying single-cell RNA sequencing to tooth development.
To highlight the utility of single-cell RNA sequencing in understanding cellular heterogeneity and developmental trajectories.
To explore the potential of this technology in prosthodontics and regeneration.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) technology.
Transcriptome analysis at the individual cell level.
Review of current literature on scRNA-seq in tooth development.

Main Results:

scRNA-seq provides a powerful tool for analyzing cellular heterogeneity.
It enables a deeper understanding of organ developmental trajectories.
This approach facilitates the identification of potential therapeutic targets.

Conclusions:

Single-cell RNA sequencing offers novel insights into the complex process of tooth development.
It is instrumental in deciphering cellular heterogeneity and developmental pathways.
The findings contribute to advancing prosthodontics and regenerative dentistry.