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Sepsis Diagnosis Based on a Parylene Matrix Chip Using LPC16:0 as a Biomarker in Comparison with Colorimetry of Total
Tae Gyeong Yun1, Jun-Hee Park1, Hye Soo Kim2
1Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, South Korea.
Abstract:
For the medical diagnosis of sepsis, it is crucial to differentiate infectious inflammation from noninfectious symptoms to prevent acute aggravation. Herein, a diagnosis for early stage sepsis was performed using LPC 16:0 and total phospholipids as small molecular biomarkers. The measurement of LPC 16:0 was conducted using a parylene matrix chip, which was developed to effectively detect small molecules in laser desorption/ionization mass spectrometry (LDI-MS). Meanwhile, the total phospholipid level was measured using colorimetry, following an enzymatic assay. Next, the two biomarkers were analyzed in serum samples from healthy volunteers, systemic inflammatory response syndrome (SIRS) patients, and sepsis patients. Diagnostic criteria were established based on the biomarker intensities observed in each patient group. After the measurements were conducted, the interference in phospholipid analysis due to hemoglobin contamination was considered. Additionally, the analytical parameters from biomarker detection were statistically interpreted and compared with those of conventional diagnostic standards. Finally, the diagnostic performance of each biomarker was evaluated by analyzing the biomarker levels between patient groups and examining their overlapping extents in box plots to distinguish sepsis from noninfectious inflammatory symptoms.
Insights
This study introduces LPC 16:0 and total phospholipids as novel biomarkers for early sepsis diagnosis. These biomarkers help differentiate infectious inflammation from noninfectious causes, improving patient outcomes.
Area of Science:
- Biochemistry
- Medical Diagnostics
- Analytical Chemistry
Background:
- Accurate sepsis diagnosis is vital to distinguish infectious from noninfectious inflammation, preventing disease progression.
- Early detection of sepsis requires reliable biomarkers for timely and effective medical intervention.
Purpose of the Study:
- To develop and validate novel small molecular biomarkers, LPC 16:0 and total phospholipids, for early-stage sepsis diagnosis.
- To differentiate infectious inflammation from noninfectious causes in sepsis patients using these biomarkers.
Main Methods:
- LPC 16:0 was measured using a parylene matrix chip with laser desorption/ionization mass spectrometry (LDI-MS).
- Total phospholipid levels were determined via colorimetry using an enzymatic assay.
- Biomarker levels were analyzed in serum samples from healthy volunteers, SIRS patients, and sepsis patients.
Main Results:
- Diagnostic criteria were established based on observed biomarker intensities across patient groups.
- The study considered and addressed potential interference in phospholipid analysis from hemoglobin contamination.
- Statistical analysis compared biomarker detection parameters with conventional diagnostic standards.
Conclusions:
- LPC 16:0 and total phospholipids show potential as effective biomarkers for early sepsis diagnosis.
- The developed method aids in distinguishing sepsis from noninfectious inflammatory conditions, crucial for appropriate medical management.
