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Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
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Efficient Strategy for Protein Drug Carrier Design for Insights into the Protein-Polyelectrolyte Interaction.
Yi-Zhen Wan1, Ning Ma1, Yu Zhang1
1State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
Langmuir : the ACS Journal of Surfaces and Colloids
|January 2, 2025
Summary
This study introduces a novel method combining ordered porous layer interferometry (OPLI) and electrophoretic light scattering (ELS) to optimize polyelectrolyte-based protein drug carriers. The findings reveal how surface charge and pH influence protein adsorption, enhancing drug loading efficiency.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Polyelectrolyte-based protein carriers are vital in drug development.
- Traditional methods lack insight into carrier fabrication and protein loading dynamics.
- A comprehensive understanding of polyelectrolyte-protein interactions is needed.
Purpose of the Study:
- To develop and validate a combined OPLI and ELS strategy for studying polyelectrolyte-protein interactions.
- To investigate the mechanism of polyelectrolyte-protein interaction during carrier fabrication and protein loading.
- To optimize protein loading efficiency for polyelectrolyte-based drug carriers.
Main Methods:
- Utilized layer-by-layer assembly to create polyelectrolyte complex (PEC) layers on silica colloidal crystal (SCC) films and nanospheres.
- Employed ordered porous layer interferometry (OPLI) for real-time analysis of PEC-protein interactions on SCC films.
- Applied electrophoretic light scattering (ELS) to measure ζ-potential for mechanistic insights and charge verification.
Main Results:
- Demonstrated that surface charge, influenced by pH and the number of polyelectrolyte layers, affects electrostatic adsorption.
- Showed a significant increase in protein adsorption with a greater ζ-potential difference between the PEC layer and proteins.
- Identified that adjusting pH during protein loading maximizes loading efficiency.
Conclusions:
- The combined OPLI and ELS approach offers an efficient strategy for developing advanced polyelectrolyte-based protein drug carriers.
- This method allows in situ interface studies to be transferred to dispersed nanoparticle systems.
- Optimizing pH and understanding charge dynamics are key to maximizing protein drug carrier efficiency.
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