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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

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High-throughput, combinatorial droplet generation by sequential spraying.

Rena Fukuda¹, Nate J Cira¹

¹Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, USA. njc222@cornell.edu.

|January 2, 2025

Summary

Sequential spraying of fluorophore-encoded solutions creates combinatorial droplets for high-throughput biochemical assays. This novel method effectively analyzes antibiotic interactions by quantifying mixed droplet concentrations over time.

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Area of Science:

Biochemistry
Biotechnology
Analytical Chemistry

Background:

Bulk and microfluidic emulsion methods advance biochemical assays.
Spray-based techniques offer rapid droplet generation but are underutilized due to random, polydisperse droplets.

Purpose of the Study:

To present a novel spray-based method for generating combinatorial droplets.
To leverage droplet polydispersity for high-throughput biochemical analysis.
To investigate pairwise antibiotic interactions using this new technique.

Main Methods:

Sequential spraying of fluorophore-encoded solutions.
Generation of mixed droplets to populate a combinatorial space.
Quantification of droplet concentrations and imaging over time.

Main Results:

Demonstrated a method for creating combinatorial droplets with quantifiable concentrations.
Successfully applied the technique to investigate antibiotic interactions.
Showcased the utility of spray-based methods for complex biological assays.

Conclusions:

Sequential spraying offers a viable approach to high-throughput biochemical assays.
This method enables the study of synergistic and antagonistic interactions.
Leveraging droplet polydispersity expands the capabilities of spray-based assays.