In Vivo Clonal Analysis Using MADM with Spatiotemporal Specificity
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Updated: May 7, 2025
Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
Published on: May 8, 2020
Yuwei Peng1,2,3, Efan Wang1,3, Zhongfu Shen1,2,3
1IDG/McGovern Institute of Brain Research, Tsinghua University, Beijing, People's Republic of China.
Abstract:
Mosaic analysis with double markers (MADM) is a powerful in vivo lineage tracing technique. It utilizes Cre recombinase-dependent interchromosomal recombination to restore the stable expression of two fluorescent proteins sparsely in individual dividing stem or progenitor cells and their progenies. Here, we describe the application of this technique for quantitative lineage analysis of radial glial progenitors in the developing mouse neocortex at the single-cell resolution.
