Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Position-effect Variegation02:32

Position-effect Variegation

6.2K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.2K
Cellular Differentiation00:57

Cellular Differentiation

2.5K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
2.5K
Incomplete Dominance01:43

Incomplete Dominance

20.2K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
20.2K
Determination01:51

Determination

16.5K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
16.5K
Lineage Commitment01:21

Lineage Commitment

2.9K
Commitment is the  process whereby stem cells:
2.9K
Punnett Squares01:00

Punnett Squares

108.6K
Overview
108.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NG2-Glia Heterogeneity Across Cortical Layers.

Glia·2025
Same author

Genetic Tracing of Progenitors from Embryo to Postnatal Brain.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering.

Biological research·2024
Same author

Spatial organization of astrocyte clones: The role of developmental progenitor timing.

Glia·2024
Same author

Cell Fate of Retinal Progenitor Cells: In Ovo UbC-StarTrack Analysis.

International journal of molecular sciences·2022
Same author

Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers.

Cells·2021
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 7, 2025

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
07:48

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

17.3K

StarTrack: Mapping Cellular Fates with Inheritable Color Codes.

M Figueres-Oñate1, Jorge García-Marqués2, A C Ojalvo-Sanz2

  • 1Instituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain. mfigueres@cajal.csic.es.

Methods in Molecular Biology (Clifton, N.J.)
|January 2, 2025
PubMed
Summary
This summary is machine-generated.

StarTrack is a genetic tool that uses a unique color code to track neural progenitor cells (NPCs) and their lineage. This method enables precise analysis of cellular fates and progenitor potential in neural development research.

Keywords:
Cell fateClonal analysisGenetic taggingIn utero electroporationLineage tracingProgenitor potentialStarTrack

More Related Videos

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
06:30

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

9.9K
Single Cell Fate Mapping in Zebrafish
07:53

Single Cell Fate Mapping in Zebrafish

Published on: October 5, 2011

13.4K

Related Experiment Videos

Last Updated: May 7, 2025

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
07:48

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

17.3K
Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
06:30

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

9.9K
Single Cell Fate Mapping in Zebrafish
07:53

Single Cell Fate Mapping in Zebrafish

Published on: October 5, 2011

13.4K

Area of Science:

  • Developmental Biology
  • Genetics
  • Neuroscience

Background:

  • Understanding cellular origins and lineages is crucial for developmental biology.
  • Neural progenitor cells (NPCs) are key to brain development, but tracing their lineage is challenging.

Purpose of the Study:

  • To introduce StarTrack, a novel multicolor genetic tool for retrospective clonal analysis of neural progenitor cells.
  • To enable precise and comprehensive tracking of cellular fates and progenitor potentials.

Main Methods:

  • StarTrack utilizes the PiggyBac system for stochastic integration of 12 distinct plasmids encoding six fluorescent proteins.
  • The system creates a unique, inheritable 'color code' within NPCs for lineage tracing.
  • Versatility is enhanced by combining multiple promoters and using fluorescent integrable constructs or PiggyBac transposase.

Main Results:

  • StarTrack allows for detailed mapping of cellular lineages originating from NPCs.
  • The technique provides insights into progenitor potentials and cellular fates.
  • Enables lineage tracing from progenitors of diverse origins.

Conclusions:

  • StarTrack offers a powerful and versatile method for neural lineage tracing.
  • This tool significantly advances the understanding of neural development and cellular origins.
  • Provides a valuable resource for researchers in developmental neuroscience.