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Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

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Related Experiment Video

Updated: May 7, 2025

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

Lineage Analysis at Single-Cell Resolution by Twin-Spot MARCM with Lineage-Restricted Drivers.

Angelica Previero^1,2, Ching-Po Yang^1,3, Tzumin Lee^4,5,6

¹Life Science Institute, University of Michigan, Ann Arbor, MI, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 2, 2025

Summary

This summary is machine-generated.

This study introduces a new lineage tracing method for Drosophila neural stem cells. It uses lineage-specific drivers to precisely map cell fate and development in complex tissues.

Keywords:

Binary sister fate decision Drosophila brain development Lineage specificity Mosaic labeling Neuronal birth order Single-cell lineage reconstruction Twin-spot MARCM

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Last Updated: May 7, 2025

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Area of Science:

Developmental Biology
Neuroscience
Genetics

Background:

Cell lineage analysis is crucial for understanding cell fate specification and diversification.
Mosaic analysis with repressible cell markers (MARCM) is a powerful technique for labeling daughter cells from a common precursor.

Purpose of the Study:

To describe detailed methods for lineage analysis in Drosophila neural stem cells.
To develop an innovative lineage tracing method for resolving complex tissue development.

Main Methods:

Utilizing twin-spot MARCM with dual repressible markers.
Employing lineage-restricted drivers to immortalize gene expression in specific lineages.
Applying methods to Drosophila neural stem cell lineages.

Main Results:

Demonstrated the power of twin-spot MARCM in subdividing complex lineages.
Successfully performed lineage analysis in Drosophila neural stem cells using lineage-specific drivers.

Conclusions:

The described lineage tracing method effectively resolves complex tissue development.
This approach enhances the systematic subdivision of complex cell lineages.