Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Specificity of gene expression in adipocytes.

K M Zezulak, H Green

Molecular and Cellular Biology

|February 1, 1985

Summary

The study found that while three specific messenger RNAs (mRNAs) are highly abundant in differentiating fat cells (adipocytes), they are not exclusive to them. However, the combination of these mRNAs is largely specific to adipocytes, indicating their role in fat cell characteristics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Challenges and opportunities for real-world evidence in clinical oncology-a view from the UK: proceedings of a national workshop.

ESMO real world data and digital oncology·2026

Same author

Best Practice for Patient-centred Radiotherapy in Clinical Trials and Beyond-A National Multidisciplinary Consensus.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025

Same author

Time to STEP UP: methods and findings from the development of guidance to help researchers design inclusive clinical trials.

BMC medical research methodology·2024

Same author

Impact of infection prevention and control practices, including personal protective equipment, on the prevalence of hospital-acquired infections in acute care hospitals during COVID-19: a systematic review and meta-analysis.

The Journal of hospital infection·2024

Same author

Improving paediatric antimicrobial stewardship in hospital-based settings: why, where and how?

JAC-antimicrobial resistance·2021

Same author

A nozzle for high-density supersonic gas jets at elevated temperatures.

The Review of scientific instruments·2018

Area of Science:

Cell biology
Molecular biology
Biochemistry

Background:

Adipose cells (adipocytes) play a crucial role in energy storage and metabolism.
Understanding the molecular basis of adipocyte differentiation is key to metabolic research.
Gene expression patterns define cell-type specific functions.

Purpose of the Study:

To investigate the cell-type specificity of three highly abundant messenger RNAs (mRNAs) during adipocyte differentiation.
To determine if these mRNAs are unique to adipose cells or also present in other tissues.
To elucidate the genetic basis of the adipocyte phenotype.

Main Methods:

Differentiating preadipose 3T3 cells into adipose cells in vitro.
Quantifying the abundance of three specific mRNAs during differentiation.
Measuring mRNA levels in various non-adipose mouse tissues.

Main Results:

The abundance of three specific mRNAs increased significantly during 3T3 cell differentiation into adipocytes.
None of the three mRNAs were exclusively found in adipocytes; they were detectable in other tissues.
The combined expression pattern of the three mRNAs showed considerable specificity for adipocytes.

Conclusions:

The distinctive characteristics of adipocytes result from the enhanced expression of multiple genes.
These genes are not exclusively expressed in adipocytes but show a specific enrichment in these cells.
The study provides insights into the molecular signature defining adipocyte identity.

Related Experiment Videos