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Human c-fms proto-oncogene: comparative analysis with an abnormal allele
Molecular and Cellular Biology
|February 1, 1985
Summary
The human c-fms proto-oncogene organization was mapped, revealing discontinuous gene sequences. An abnormal allele showed a deletion near a c-fms exon, impacting genetic structure.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- The c-fms proto-oncogene is crucial in cellular processes.
- Understanding its genetic organization is key to oncogenesis research.
- Abnormalities in proto-oncogenes can lead to cancer development.
Purpose of the Study:
- To determine the genomic organization of the human c-fms proto-oncogene.
- To compare the normal c-fms locus with an abnormal allele.
- To identify genetic alterations in the abnormal c-fms locus.
Main Methods:
- DNA sequencing analysis
- Gene mapping
- Comparative genomics
Main Results:
- Human c-fms homologous sequences span approximately 32 kilobase pairs.
- These sequences are discontinuously and colinearly arranged with the viral oncogene.
- The abnormal c-fms locus features a 426 base pair deletion in its 3' region, near a putative exon.
Conclusions:
- The genomic structure of the human c-fms proto-oncogene has been elucidated.
- A specific deletion in the 3' portion of an abnormal c-fms allele was identified.
- This finding provides insights into the genetic basis of c-fms related abnormalities.