Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A NACHT domain-containing protein Ncp is required for appendage-associated unconventional protein secretion in the fungus <i>Penicillium herquei</i>.

iScience·2026

Same author

Methyl dichloroasterrate alleviates lipopolysaccharide-induced neuroinflammation and depression-like behaviors in mice by upregulating FXR expression.

International immunopharmacology·2026

Same author

Machine Learning Estimation of Gestational Age at Delivery Using Linked Mother-Infant Electronic Health Records Across Two Health Systems.

medRxiv : the preprint server for health sciences·2026

Same author

A phenotype-to-mechanism framework links phenome-wide comorbidity architecture to molecular mechanisms and therapeutic discovery in complex diseases.

medRxiv : the preprint server for health sciences·2026

Same author

Ultrasensitive electrochemical sensor for carbendazim in juices using hierarchical single-walled carbon-based nanotubes/nanohorns hybrid conductive networks integrated with stellate mesoporous silica nanospheres.

Food chemistry·2026

Same author

Corrective osteotomy for distal radius malunion using 3D-printed patient-specific guides and spacers: a retrospective comparative study.

BMC musculoskeletal disorders·2026

Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026

Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026

Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026

Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026

Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026

Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 13, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

ImageDoubler: image-based doublet identification in single-cell sequencing.

Kaiwen Deng¹, Xinya Xu², Manqi Zhou¹

¹Gilbert S. Omenn Department of Computational Medicine & Bioinformatics, University of Michigan, Ann Arbor, MI, USA.

Nature Communications

|January 2, 2025

Summary

This summary is machine-generated.

ImageDoubler, an image-based model, effectively detects doublets in single-cell sequencing data. This method surpasses traditional genomic approaches, improving data accuracy and interpretation.

More Related Videos

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Published on: May 27, 2020

Related Experiment Videos

Last Updated: May 13, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Published on: May 27, 2020

Area of Science:

Genomics
Bioinformatics
Cell Biology

Background:

Single-cell sequencing is crucial for understanding cellular heterogeneity.
Doublets, where multiple cells are co-sequenced, compromise data integrity.
Current doublet detection methods often rely on simulated data, limiting accuracy.

Purpose of the Study:

To introduce ImageDoubler, an image-based model for identifying doublets and missing samples in single-cell sequencing.
To evaluate the efficacy of ImageDoubler compared to existing genomic-based methods.

Main Methods:

Developed an innovative image-based model, ImageDoubler.
Leveraged Fluidigm single-cell sequencing image data for doublet detection.
Compared ImageDoubler's performance against traditional genomic-based doublet detection techniques.

Main Results:

ImageDoubler achieved a doublet detection rate of up to 93.87%.
Demonstrated a minimum improvement of 33.1% in F1 scores compared to genomic methods.
Highlighted the effectiveness of image data in doublet identification.

Conclusions:

ImageDoubler offers a robust alternative for doublet detection in single-cell sequencing.
Image-based approaches can overcome limitations of genomic-based doublet detection methods.
Accurate doublet identification is essential for reliable single-cell data interpretation.