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Updated: Jun 4, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
The Hira complex regulates Gli3R-dependent transcription in Hedgehog signaling and medulloblastoma cell growth and
Weronika Skarżyńska1,2, Brygida Baran1,2, Paweł Niewiadomski3
1Centre of New Technologies, University of Warsaw, Warsaw, Poland.
Abstract:
Regulation of the Hedgehog pathway activity may be supported by coactivators and corepresors of its main effectors- Gli transcription factors. While activation processes are well studied, repression mechanisms remain elusive. We identified chromatin remodelling complex Hira to interact with Gli3R protein, showed that its loss-of-function changes Hh pathway activity, and examined possible mechanism behind the observed effect. We also established that Hira influences the viability and migratory abilities of Hh-dependent medulloblastoma Daoy cells. Our study paves the way for a better understanding of processes involved in Hh pathway regulation and Hh-dependent carcinogenesis.
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