Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

4.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.7K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

712
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
712
Proteomics01:33

Proteomics

7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-Zika Virus Activity of Azvudine via Inhibition of RNA Replication by Its Active Triphosphate Form.

Journal of medical virology·2026
Same author

The rising power of females: Dioecious shrub enhances soil organic carbon sequestration via fungal necromass in chronosequence of desertified alpine grassland restoration.

Journal of environmental management·2026
Same author

Adsorption-catalysis integrated Au@ZIF-8 for structural detoxification and trace detection of nitro impurities.

Nanoscale·2026
Same author

Physiology-Based Pharmacokinetic Modeling of Ropivacaine After External Oblique Intercostal Plane Block in Open Liver Surgery Patients.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Comparative clinical study of total thoracoscopic surgery and thoracoscopy-assisted small-incision surgery for multiple rib fractures.

Scientific reports·2026
Same author

M2pep-modified liposomal nanoparticles delivering siITGB4 induce apoptosis and inhibit NSCLC metastasis via macrophage reprogramming.

Apoptosis : an international journal on programmed cell death·2026

Related Experiment Video

Updated: Jun 4, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.7K

Enalomics: A Mass Spectrometry-Based Approach for Profiling, Identifying, and Semiquantifying Enals in Biological

Congyu Ma1, Wen Zhang2, Jialong Jing1

  • 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P. R. China.

Analytical Chemistry
|January 3, 2025
PubMed
Summary

Researchers developed "enalomics," a mass spectrometry method to identify and quantify reactive aldehydes called enals. This approach identified new enals and potential biomarkers for myocardial infarction, aiding disease diagnosis.

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K

Related Experiment Videos

Last Updated: Jun 4, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.7K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Pathology

Background:

  • Aldehydes from lipid peroxidation (LPO) impact cellular processes and are linked to diseases.
  • Enals, reactive aldehydic metabolites, are implicated in neurodegeneration, cancer, and cardiovascular diseases.
  • Endogenous enals are poorly characterized due to instability and low abundance.

Purpose of the Study:

  • To introduce and validate a mass spectrometry-based approach, "enalomics," for profiling endogenous enals.
  • To identify and semiquantify enals in biological samples.
  • To assess the diagnostic potential of enals in myocardial injury and infarction.

Main Methods:

  • Developed "enalomics" using mass spectrometry (MS) with derivatization (2,4-dinitrophenylhydrazine) and ascorbic acid.
  • Employed precursor ion scanning, dynamic multiple reaction monitoring, and high-resolution MS for enal identification.
  • Utilized retention time and carbon number correlations for reliable identification and quantification.

Main Results:

  • Identified 157 enals in rat tissues (127 novel) and 29 in human plasma (24 novel).
  • Demonstrated altered enal metabolism in rats with myocardial injury.
  • Identified 14 plasma enal biomarkers for myocardial infarction (MI) with high diagnostic accuracy (AUC ≥ 0.8603).

Conclusions:

  • Enalomics provides a comprehensive method for analyzing endogenous enals in biological systems.
  • Enal metabolism is significantly altered in myocardial injury and infarction.
  • Plasma enals show promise as sensitive biomarkers for early diagnosis and intervention in MI patients.