Related Experiment Video
Updated: May 7, 2025
09:12
Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
Published on: May 3, 2021
2.5K
Structural Dynamics of Neutral Amino Acid Transporter SLC6A19 in Simple and Complex Lipid Bilayers
Budheswar Dehury1,2, Sarbani Mishra2, Sunita Panda2
1Department of Bioinformatics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.
Journal of Cellular Biochemistry
|January 3, 2025
Summary
Molecular dynamics simulations reveal how lipids affect the B0AT1 transporter's structure and function. This provides insights into amino acid metabolism disorders and potential drug targets.
Area of Science:
- Biochemistry
- Structural Biology
- Computational Biology
Background:
- B0AT1 (SLC6A19) is a key neutral amino acid transporter.
- Its function is crucial for amino acid metabolism but its structure-function in lipids is poorly understood.
Purpose of the Study:
- To investigate the structural characteristics and dynamics of B0AT1 within lipid bilayers.
- To understand the influence of different lipid environments on B0AT1 stability and function.
Main Methods:
- All-atoms molecular dynamics (MD) simulations.
- Simulations performed in simplified (POPC) and complex native-like lipid bilayers.
- Structure-based protein stability analysis.
Main Results:
- B0AT1 exhibits enhanced structural stability and features in complex lipid environments compared to POPC.
- Specific phospholipids alter B0AT1's pore radius, hydrophobic index, and surface charge, impacting helix flexibility.
- Identified key lipid-binding residues and lipid-mediated structural deviations affecting substrate binding sites.
Conclusions:
- POPC bilayers serve as a valid baseline for initial SLC transporter studies.
- The study provides a framework for understanding B0AT1 in realistic membrane environments.
- Findings offer potential for developing new therapeutic agents targeting B0AT1.
Related Concept Videos
01:31Membrane Transporters
9.9K
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
9.9K
01:19Membrane Asymmetry Regulating Transporters
4.2K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.2K
01:44The Significance of Membrane Transport
19.7K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
19.7K
01:16Facilitated Diffusion
252
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
252
01:19Drug Absorption Mechanism: Carrier-Mediated Membrane Transport
3.2K
Certain large, lipid-insoluble drug molecules that resemble amino acids, peptides, or glucose, require specialized carrier proteins to facilitate their diffusion across cell membranes. This transport can occur through either facilitated diffusion, which does not require energy input, or active transport, which does require energy input.
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
3.2K
01:32Secondary Active Transport
6.6K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
6.6K