Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Recent progress and policy recommendations for biomanufacturing technology in China].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026

Same author

Photocatalytic C-3 Acylation of Indoles Using ICF<sub>2</sub>SO<sub>2</sub>Ph as Acyl Fluoride Precursor.

Organic letters·2026

Same author

Scaling covalent ligand discovery through dynamic combinatorial library-versus-proteome screening.

Nature communications·2026

Same author

Thiol-Retaining N-Terminal Cysteine Chemistry for Dual Modification and Bicyclic Peptide Construction.

Journal of the American Chemical Society·2026

Same author

Disulfide-Directed Multicylic Peptides for Targeting and Radionuclide Imaging of HER2 Expressing Tumors.

Journal of medicinal chemistry·2026

Same author

TROP2-targeted NIR-II fluorescence imaging for visualizing surgical margins and metastatic sentinel lymph nodes in breast cancers.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Linker Engineering toward NIR-II Metal-Organic Framework with Maximal Emission beyond 1000 nm for Inflammatory Bowel Disease Imaging.

Journal of the American Chemical Society·2026

Same journal

Observing Kinetic Selectivity in Anthracene Photodimerization through Selective Quenching by Excited States of Proximate Rare Earth Cations.

Journal of the American Chemical Society·2026

Same journal

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026

Same journal

Large Thermo- and Mechanosalient Actuation via Cooperative Twist Elasticity-Induced Packing Motif Conversion.

Journal of the American Chemical Society·2026

Same journal

Discovery and Biosynthesis of Lanthipeptides Featuring an Azepinoindole Scaffold by Radical <i>S</i>-Adenosylmethionine Enzyme-Catalyzed C-C Bond Formation.

Journal of the American Chemical Society·2026

Same journal

Enantiopurity-Controlled Magnetism in a Two-Dimensional Organic-Inorganic Material.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2025

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

A Single Bioorthogonal Reaction for Multiplex Cell Surface Protein Labeling.

Yang Huang^1,2, Chengyang Wu³, Anjing Lu²

¹School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

Journal of the American Chemical Society

|January 3, 2025

Summary

This summary is machine-generated.

New 2-[(alkylthio)(aryl)methylene]malononitrile (TAMM) molecules enable fast, site-specific protein labeling for advanced multicolor fluorescence imaging in cells and living animals.

More Related Videos

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

Related Experiment Videos

Last Updated: Jun 4, 2025

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

Area of Science:

Bioconjugation Chemistry
Molecular Imaging
Chemical Biology

Background:

Small-molecule fluorophores are essential for fluorescence imaging.
Current protein conjugation methods limit multicolor imaging applications.

Purpose of the Study:

To develop novel molecules for efficient and site-specific protein labeling.
To overcome limitations in multicolor fluorescence imaging.

Main Methods:

Mechanistic investigation of 2-[(alkylthio)(aryl)methylene]malononitrile (TAMM) reactions with 1,2-aminothiol.
Application of TAMM condensation for site-specific labeling in cell lines, primary neurons, and living mice.
Integration with genetic code expansion and proteolytic cleavage for iterative protein modification.
Compatibility with click chemistry reactions (CuAAC, tetrazine ligation) for multiplexing.

Main Results:

Identified TAMM molecules with labeling rates exceeding 10^4 M^-1 s^-1.
Achieved site-specific labeling of surface proteins under mild conditions.
Demonstrated selective modification of three cell surface proteins iteratively.
Enabled four-color fluorescent labeling by combining TAMM with other bioconjugation strategies.

Conclusions:

TAMM condensation offers a rapid and versatile platform for bioconjugation.
This method significantly advances site-specific, multicolor protein imaging capabilities.
Bioconjugation chemistry limitations are overcome for multiplex protein analysis.