Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Infrared (IR) Spectroscopy: Overview

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiplexed optoacoustic tracking and magnetic actuation of labeled blood cells in living mice.

Science advances·2026

Same author

Fully cove-edged bilayer nanographene with parallel displaced stacking.

Nature communications·2026

Same author

NIR-II fluorescence projection technology for augmented-reality surgical navigation.

National science review·2026

Same author

Cationic Polymer-Photothermal Agent Conjugates with Enhanced Light Absorption and Tumor Accumulation for Efficient Phototherapy of Osteosarcoma.

ACS nano·2026

Same author

Logic-Gated NIR-IIb Theranostic Nanozymes for Longitudinal Quantitative Assessment of In-Situ Cytotoxic T Lymphocytes.

Analytical chemistry·2026

Same author

Hundred-Nanosecond Equivalent Pixel Dwell Time for Deep-Tissue 3D Three-Photon Fluorescence Microscopy via Sparse X-Y-Z Reconstruction.

Small methods·2026

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

High-Definition, Video-Rate Triple-Channel NIR-II Imaging Using Shadowless Lamp Excitation and Illumination.

Yuhuang Zhang¹, Shiyi Peng¹, Jian Guo²

¹State Key Laboratory of Extreme Photonics and Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, China.

|January 3, 2025

Summary

This summary is machine-generated.

A new multichannel imaging system using the second near-infrared window (NIR-II) provides clear, real-time surgical navigation. This video-rate system simplifies light sources, enhancing safety and reducing crosstalk for improved surgical visualization.

Keywords:

NIR-II bioimaging multichannel imaging quantum dot shadowless lamp surgical navigation

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jul 3, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Area of Science:

Biomedical Optics
Medical Imaging
Surgical Navigation

Background:

Multichannel imaging in the second near-infrared (NIR-II) window is crucial for complex surgical environments.
Existing methods often lack simplicity, high quality, video-rate capability, or have safety concerns.
A need exists for a safe, efficient, and high-performance multichannel imaging solution for surgery.

Purpose of the Study:

To develop a simple, high-quality, video-rate multichannel imaging method for surgical navigation.
To reduce safety risks associated with excitation light sources in surgical imaging.
To achieve real-time, high-performance imaging with minimal crosstalk in the NIR-II window.

Main Methods:

Developed a triple-channel imaging system utilizing the NIR-IIx window (1400-1500 nm) and NIR-IIc window (1000-1100 nm and 1700-1880 nm).
Implemented an in vivo multichannel imaging-assisted surgical navigation mode.
Utilized standard shadowless lamp excitation and illumination, eliminating the need for extra excitation light sources.

Main Results:

Achieved exceptional clarity in triple-channel imaging across the specified NIR-II bands.
Demonstrated an impressive signal-to-crosstalk ratio as high as 22.10.
Operated the system at a video frame rate of 25 frames per second (fps) under ambient illumination.

Conclusions:

The developed system offers a practical fluorescence surgical navigation paradigm.
Provides a reference for real-time, high-imaging-performance multichannel imaging with minimal crosstalk.
Simplifies light sources and lowers safety risks for enhanced surgical visualization.