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FAM107A Inhibits the Growth, Invasion and Aerobic Glycolysis of LUAD Cells by Regulating CRYAB/PI3K/AKT

Fei Ming1, DaiPing Zhang2

  • 1Department of the Thoracic Surgery, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 116 Zhuodaoquan South Road, Hongshan District, Wuhan, 430070, Hubei, China. Mingfei_666@163.com.

Biochemical Genetics
|January 3, 2025
PubMed

Insights

The tumor suppressor FAM107A inhibits lung adenocarcinoma (LUAD) progression by suppressing cell growth, invasion, and aerobic glycolysis via the CRYAB/PI3K/AKT pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lung adenocarcinoma (LUAD) is an aggressive cancer with limited treatment options.
  • The tumor suppressor gene FAM107A shows potential in various cancers, but its role in LUAD is not well-defined.

Purpose of the Study:

  • To investigate the function of FAM107A in LUAD.
  • To elucidate the molecular mechanisms underlying FAM107A's effects on LUAD progression and metabolism.

Main Methods:

  • Gene expression analysis to assess FAM107A levels in LUAD.
  • Cellular assays to evaluate the impact of FAM107A overexpression on LUAD cell growth and invasion.
  • Metabolic assays to determine effects on aerobic glycolysis.
  • Western blotting to analyze signaling pathway activation (CRYAB/PI3K/AKT).
  • In vivo mouse models to validate findings.

Main Results:

  • FAM107A is significantly downregulated in LUAD tissues.
  • Overexpression of FAM107A suppressed LUAD cell proliferation and invasion.
  • FAM107A overexpression reduced aerobic glycolysis in LUAD cells.
  • Mechanistically, FAM107A was found to regulate the CRYAB/PI3K/AKT signaling pathway.
  • In vivo studies confirmed FAM107A's tumor-suppressive effects.

Conclusions:

  • FAM107A acts as a tumor suppressor in LUAD.
  • FAM107A inhibits LUAD progression by modulating the CRYAB/PI3K/AKT pathway and aerobic glycolysis.
  • FAM107A represents a potential therapeutic target for lung adenocarcinoma treatment.
Keywords:
CRYABCarbohydrate metabolismFAM107ALung adenocarcinoma (LUAD)PI3K/AKT signaling

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