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Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method.
Marlee Shaffer1, Devin North1, Kyle Bibby2
1Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA.
Food and Environmental Virology
|January 4, 2025
Summary
A new Nanotrap Microbiome Particles (NMP) method for wastewater surveillance shows promise. While NMP concentration varies by target, it increases detection of unique viral species in wastewater samples.
Area of Science:
- Environmental microbiology
- Virology
- Public health surveillance
Background:
- Wastewater-based surveillance is crucial for monitoring community infectious diseases.
- Current viral concentration methods in wastewater analysis have limitations in time, cost, and efficiency.
- Optimizing concentration techniques is vital for sensitive and accurate pathogen detection.
Purpose of the Study:
- To evaluate the concentration efficiency and bias of Nanotrap Microbiome Particles (NMP) for viral detection in wastewater.
- To compare NMP performance against direct extraction methods for various viral and bacterial indicators.
- To assess the utility of NMP for comprehensive viral community profiling in wastewater.
Main Methods:
- Wastewater samples were processed using Nanotrap Microbiome Particles (NMP) for viral concentration.
- Concentration efficiency was assessed using specific viral (Carjivirus, Pepper Mild Mottle Virus) and bacterial (HF183) indicators.
- Targeted metagenomic sequencing was employed to analyze viral species detected by NMP and direct extraction.
Main Results:
- NMP demonstrated target-specific concentration efficiencies, with lower recovery for HF183 and Carjivirus but significantly higher recovery for Pepper Mild Mottle Virus compared to direct extraction.
- NMP yielded significantly more unique species reads per sample than direct extraction, detecting previously undetected species.
- Key viral families identified included Adenoviridae, Caliciviridae, Herpesviridae, Papillomaviridae, and Polyomaviridae.
Conclusions:
- Nanotrap Microbiome Particles (NMP) offer a novel approach for concentrating viruses in wastewater.
- NMP exhibits differential concentration abilities for various viral targets, necessitating optimization for specific applications.
- The enhanced detection of unique viral species by NMP suggests its potential for improving wastewater-based infectious disease surveillance.
Keywords:
MetagenomeNanotrap Microbiome ParticlesViral concentrationViromeWastewater-based epidemiology
