Related Experiment Video
Updated: Jun 4, 2025

Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development
Published on: March 17, 2023
Optimization of hypobaric and ultrasonic processing of persimmon rhamnogalacturonan-I to enhance drug-digestion
Julio Salazar-Bermeo1, Bryan Moreno-Chamba1, Marta Hernández-García2
1Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández, 03202 Elche, Alicante, Spain; Instituto Universitario de Ingeniería de Alimentos-FoodUPV, Universitat Politècnica de València, Avenida Fausto Elio s/n, Edificio 8E, Acceso F Planta 0, 46022 Valencia, Spain.
Abstract:
The biological activity of polysaccharides used for nutraceuticals/drug excipients has been a neglected area of study. This work deals with the preparation, optimization, characterization, and evaluation of persimmon (Diospyros kaki Thunb.) fruit by-products and the study of the resultant dietary fiber (DF) interaction with other compounds, using acetaminophen as a model. Processing conditions for persimmon by-products were optimized to enhance antioxidant activity, with hypobaric, ultrasonic, and drying conditions tested at three levels of time and pH. The optimized DF was evaluated through in-vitro and ex-vivo release and permeation studies. Optimal conditions included three cycles of vacuum instantaneous expansion coupled with ultrasound waves (USEX), 42 min of ultrasound assisted extraction (UAE), and a pH of 1.5. After treatments, the antioxidant capacity (AC) increased six-fold, and zeta potential (ζ) analysis indicated polysaccharide aggregation at the optimized pH. The optimized polysaccharides, mainly formed by rhamnogalacturonan-I, displayed nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent activity. In-vitro drug-DF interaction studies showed higher acetaminophen release during digestion. Permeation kinetics adhered to the Korsmeyer-Peppas model in both ex-vivo and in-vitro models, suggesting complex permeation mechanisms. Results suggest that the optimized DF enhances the bioavailability and controlled release of acetaminophen, indicating its potential for use in drug delivery systems and nutraceutical applications.
More Related Videos
Related Concept Videos
Factors Influencing Drug Absorption: Pharmaceutical Parameters
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
Factors Affecting Dissolution: Particle Size and Effective Surface Area
Factors Influencing Drug Absorption: Physicochemical Parameters
Enhanced drug absorption can be achieved by reducing particle sizes and increasing surface areas, thereby facilitating...
Factors Influencing Drug Absorption: Drug Dissolution
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...

