Preparation of Cello-Oligosaccharides by Precise-Controlled Enzymatic Depolymerization and Its Amphiphilic...

Area of Science:

• Biotechnology and Biomaterials
• Food Science and Technology
• Chemical Engineering

Background:

• Cello-oligosaccharides (COS) exhibit prebiotic properties beneficial for gut health.
• Developing novel functional ingredients from biomass is crucial for sustainable applications.

Purpose of the Study:

• To produce cello-oligosaccharides (COS) with specific chain lengths via enzymatic hydrolysis.
• To synthesize and characterize amphiphilic COS (SAC12) for enhanced emulsifying properties.
• To evaluate the stability and characteristics of SAC12-stabilized emulsions.

Main Methods:

• Enzymatic hydrolysis of cellulose using Ultimase BWL 40 and Celluclast 1.5 L to produce COS.
• Alkylsuccinylation of COS to create amphiphilic SAC12.
• Characterization using FTIR and 1HNMR spectroscopy.
• Emulsion preparation and stability assessment (droplet size, PDI, zeta potential).

Main Results:

• Enzymatic hydrolysis yielded COS predominantly composed of 4-10 glucose units.
• Successful synthesis of amphiphilic SAC12 confirmed by spectroscopic methods.
• SAC12 stabilized 70 wt% fish oil-in-water emulsions with nano-/microdroplets (500-540 nm) and narrow PDI (<0.1).
• Emulsions exhibited high stability with a strongly negative zeta potential (-45 to -48 mV).

Conclusions:

• Alkylsuccinylated COS (SAC12) demonstrates potent cosurfactant-free emulsifying capabilities.
• SAC12 offers a sustainable and versatile ingredient for nutraceutical and pharmaceutical industries.
• The study highlights the potential of modified COS in advanced material applications.