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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
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Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem

Mikhail Vokuev1, Artem Melekhin2, Anastasia Frolova1

  • 1Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.

Food Chemistry: X
|January 6, 2025
PubMed

Insights

This study monitored metronidazole (MNZ) and its metabolite in chicken eggs stored for 3 months. Results show analyte stability in both refrigerated and frozen conditions, crucial for food safety monitoring.

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Pharmacology

Background:

  • Metronidazole (MNZ) is widely used in the food industry, necessitating monitoring due to antimicrobial resistance risks.
  • Legal and regulatory requirements demand reliable analysis of food samples, even after prolonged storage.
  • Analyte stability during storage is critical for accurate quantification and legal defensibility.

Purpose of the Study:

  • To validate an HPLC-MS/MS method for analyzing metronidazole and its metabolite in chicken eggs.
  • To assess the stability of metronidazole and hydroxymetronidazole in chicken eggs stored at +4°C and -20°C for 3 months.
  • To investigate the time-dependent degradation of these analytes in stored egg samples.

Main Methods:

  • A validated High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) approach was employed.
  • Chicken eggs were fortified with metronidazole and its hydroxy metabolite by administering an analyte solution to hens.
  • Samples were stored under refrigeration (+4°C) and frozen (-20°C) conditions for 3 months.

Main Results:

  • The HPLC-MS/MS method demonstrated reliability for quantifying metronidazole and hydroxymetronidazole.
  • Analyte levels in chicken eggs remained stable over a 3-month storage period in both refrigerated and frozen conditions.
  • Degradation patterns were investigated, showing minimal loss of analytes over time.

Conclusions:

  • The validated HPLC-MS/MS method is suitable for monitoring metronidazole and its metabolite in chicken eggs.
  • Metronidazole and its hydroxy metabolite are stable in chicken eggs during extended storage at both +4°C and -20°C.
  • This stability supports the reliable analysis of metronidazole in food safety and legal contexts.
Keywords:
HydroxymetronidazoleLiquid chromatographyMetronidazoleTandem mass spectrometry

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