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Updated: Jul 6, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Automated electrochemical oxygen sensing using a 3D-printed microfluidic lab-on-a-chip system.

Daniel Kaufman¹, Steffen Winkler^2,3, Christopher Heuer^2,3

¹Nanobioelectronics Laboratory (NBEL), Department of Biomedical Engineering, Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, 8410501 Beer Sheva, Israel. benyoav@bgu.ac.il.

|January 7, 2025

Summary

This summary is machine-generated.

This study introduces a 3D-printed microfluidic device with integrated sensors to accurately measure dissolved oxygen and generate hydrogen peroxide. This technology enables real-time monitoring and regulation of oxygen levels in physiological models.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Microfluidics
Electrochemical Sensing

Background:

Standard physiological models often fail to replicate in vivo oxygen levels.
Local generation of reactive oxygen species (ROS) is frequently overlooked in current models.
Accurate monitoring of dissolved oxygen is critical for understanding physiological and pathological processes.

Purpose of the Study:

To develop a microfluidic lab-on-a-chip system for precise dissolved oxygen monitoring.
To integrate electrochemical sensing capabilities for real-time ROS generation, specifically hydrogen peroxide (H2O2).
To create a tool for validating custom electrodes and controlling oxygen levels in organ-on-chip systems.

Main Methods:

Fabrication of a microfluidic device using high-resolution 3D printing.
Integration of electrochemical dissolved oxygen sensors and a commercial optical oxygen sensor.
Inclusion of a micromixer, bubble-trap, and electrochemical cell with gold/platinum black electrodes.

Main Results:

Sensitive electrochemical oxygen monitoring with a limit of detection of 11.9 ± 0.3 μM.
Statistically significant correlation between electrochemical and optical oxygen sensor measurements.
Successful fabrication of a one-step, integrated microfluidic system.

Conclusions:

The developed microfluidic system accurately monitors physiological oxygen concentrations and generates H2O2.
This technology offers a valuable tool for characterizing electrodes and validating sensor performance.
The system holds potential for real-time regulation of oxygen and ROS in advanced organ-on-chip models.