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Core-Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity
Tatyana Kovshova1, Julia Malinovskaya1, Julia Kotova1
1Faculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, Russia.
Biomolecules
|January 8, 2025
Summary
This study compared core-shell nanoparticles with PLGA cores and various polymer shells, evaluating structural integrity and cellular uptake. Results showed all shells enhanced nanoparticle delivery to glioma cells, aiding optimal method selection.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Core-shell nanoparticles offer tunable properties for drug delivery.
- Polymer selection and shell formation methods significantly impact nanoparticle characteristics.
Purpose of the Study:
- To compare structural integrity and properties of PLGA core-shell nanoparticles with different polymer shells.
- To evaluate the influence of shell composition and formation technique on nanoparticle characteristics.
- To assess the impact of core-shell structure on cellular uptake and intracellular integrity.
Main Methods:
- Preparation of PLGA core nanoparticles using high-pressure homogenization-solvent evaporation or nanoprecipitation.
- Shell formation via adsorption, interfacial embedding, or conjugation using poloxamer 188, DIVEMA, or HSA.
- Dual fluorescent labeling for tracking and FRET analysis.
- Characterization using Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), and fluorescence spectroscopy.
- Assessment of cellular uptake and intracellular integrity in Gl261 murine glioma cells.
Main Results:
- Nanoparticle size ranged from 100-250 nm with negative zeta potentials.
- Core-shell structure confirmed by TEM and fluorescence spectroscopy, showing FRET phenomena.
- All tested polymer shells (P188, DIVEMA, HSA) enhanced cellular uptake in Gl261 cells.
- Intracellular colocalization confirmed the structural integrity of core-shell nanoparticles after cellular incubation.
Conclusions:
- The choice of shell polymer and preparation method influences core-shell nanoparticle properties and performance.
- Core-shell nanoparticles with various polymer shells demonstrate enhanced cellular uptake and maintain structural integrity.
- This study provides a framework for selecting optimal core-shell nanoparticle preparation methods for specific applications.
