Jove
Visualize
Contact Us

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

35.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.5K
DNA Helicases00:55

DNA Helicases

21.1K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.1K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.0K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.2K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.2K
Homologous Recombination02:31

Homologous Recombination

50.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of non-canonical p53 functions in DNA replication and recombination for variant classification.

Cell death & disease·2026
Same author

An Aptamer-Based gFET-Sensor for Specific Quantification of Gene Therapeutic Human Adenovirus Type 5.

Biosensors·2025
Same author

Co-expression of auxiliary genes enhances the activity of a heterologous O<sub>2</sub>-tolerant hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803.

Biotechnology for biofuels and bioproducts·2025
Same author

Compromised CDK12 activity causes dependency on the high activity of O-GlcNAc transferase.

Glycobiology·2024
Same author

Correlation of bioactive marker compounds of an orally applied <i>Morus alba</i> root bark extract with toxicity and efficacy in BALB/c mice.

Frontiers in pharmacology·2023
Same author

Toward a synthetic hydrogen sensor in cyanobacteria: Functional production of an oxygen-tolerant regulatory hydrogenase in <i>Synechocystis</i> sp. PCC 6803.

Frontiers in microbiology·2023
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

248

HROB Is Implicated in DNA Replication.

Julia Kutz1,2, Hannes Schmietendorf1,2, Sheikh Anika Rahman1,2

  • 1Project Group Biochemistry, Leibniz Institute on Aging-Fritz Lipmann Institute, D-07745 Jena, Germany.

Genes
|January 8, 2025
PubMed
Summary
This summary is machine-generated.

The newly identified HROB protein interacts with DNA replication machinery and is crucial for maintaining genomic integrity. Its expression peaks during S phase, and its ectopic expression halts DNA replication, suggesting a role in repairing DNA damage.

Keywords:
DNA interstrand crosslink (ICL) repairDNA replicationFörster resonance energy transfer (FRET)HROBprotein overexpressionsoluble protein fragment identification

More Related Videos

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.1K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

955

Related Experiment Videos

Last Updated: Jun 3, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

248
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.1K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

955

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA replication is a complex process vital for genetic information duplication.
  • Maintaining genomic integrity requires coordination between DNA damage response, repair, and replication.
  • HROB is a recently identified protein partner and activator of the Mcm8/9 helicase involved in DNA interstrand crosslink (ICL) repair.

Purpose of the Study:

  • To investigate the role of HROB in DNA replication and its association with replication machinery.
  • To understand the expression pattern of HROB during the cell cycle.
  • To elucidate the structural features and evolutionary origin of HROB.

Main Methods:

  • Flow cytometry Förster resonance energy transfer (FRET) assay to detect protein associations.
  • Analysis of HROB expression levels across the cell cycle.
  • Structural prediction and homology searches for HROB.

Main Results:

  • HROB expression is co-regulated with DNA replication factors, peaking in S phase and decreasing in quiescence.
  • HROB is an intrinsically disordered protein with a helix-rich region and an oligonucleotide/oligosaccharide-binding-fold motif.
  • FRET assays confirmed associations between HROB and DNA replication proteins.
  • Ectopic expression of HROB resulted in a near-complete shutdown of DNA replication.

Conclusions:

  • HROB plays a significant role in DNA replication, particularly in navigating challenging DNA structures like ICLs.
  • The findings suggest HROB is a key component in the cellular response to DNA damage during replication.
  • HROB's interaction with the replication machinery highlights its importance in maintaining genomic stability.