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Related Concept Videos

Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

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Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
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Renal Drug Excretion: Tubular Secretion01:28

Renal Drug Excretion: Tubular Secretion

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Active tubular secretion is a robust, energy-demanding process that utilizes carrier systems to transport drugs into renal tubules. The active renal secretion systems include the organic anion transporter (OAT) for weak acids and the organic cation transporter (OCT) for weak bases. Structurally similar drugs can compete for the same transporter, potentially leading to drug accumulation and toxicity. However, this principle can be exploited therapeutically. One example is probenecid (Probalan),...
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Renal Drug Excretion: Glomerular Filtration01:02

Renal Drug Excretion: Glomerular Filtration

137
The kidney serves as the primary organ responsible for eliminating drugs and their metabolites from the body. This process, known as renal elimination, starts with glomerular filtration and results in urine formation. Each kidney houses millions of functional units called nephrons, where urine production occurs. A nephron has two main components: a renal corpuscle and a renal tubule.
Drugs gain access to the kidney via the renal artery, which progressively branches off into afferent arterioles....
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Renal Clearance01:23

Renal Clearance

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The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
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Renal Drug Excretion: Tubular Reabsorption01:25

Renal Drug Excretion: Tubular Reabsorption

89
Tubular reabsorption, a process occurring post-glomerular filtration of drugs in the renal tubule, is a critical determinant of drug half-life. During the process of renal excretion, as the glomerular filtrate progresses to the distal convoluted tubule (DCT), drugs that are highly permeable, lipophilic, and nonionized undergo passive reabsorption from the tubular fluid into the surrounding peritubular capillaries. This reabsorption process restricts their elimination through the kidneys. This...
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Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

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The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
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Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
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Urinary GADD45G Protein Excretion Is Associated with IgA Nephropathy Progression.

Min-Jeong Lee1, Hyunee Yim2, Ji Eun Park1,3

  • 1Department of Nephrology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.

Biomedicines
|January 8, 2025
PubMed
Summary

Urinary GADD45G protein levels indicate IgA nephropathy progression. Higher GADD45G in urine correlates with worsening kidney function and protein levels, serving as a potential biomarker for disease advancement.

Keywords:
IgA nephropathychronic kidney diseasegrowth arrest and DNA damage 45G (GADD45G)kidney biomarker

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Biochemistry

Background:

  • Growth arrest and DNA damage 45G (GADD45G) proteins are key in DNA damage response and cell cycle regulation.
  • GADD45G family proteins play roles in cellular stress responses.
  • Investigating GADD45G's role in kidney diseases is crucial for understanding pathogenesis.

Purpose of the Study:

  • To investigate the association between urinary GADD45G protein levels and the progression of IgA nephropathy.
  • To determine if urinary GADD45G can serve as a predictive biomarker for renal function decline in IgA nephropathy patients.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to quantify urinary GADD45G levels in IgA nephropathy patients.
  • Serum creatinine (SCr) and urinary protein concentrations were measured.
  • Renal biopsy tissues were analyzed using immunohistochemistry for GADD45G expression.

Main Results:

  • Urinary GADD45G levels were significantly correlated with SCr-slopes and urinary protein concentrations.
  • Patients with higher urinary GADD45G tertiles exhibited a significantly higher SCr-slope, indicating faster renal function deterioration.
  • Urinary GADD45G demonstrated good performance in predicting renal outcomes, with a negative predictive value of 100%.

Conclusions:

  • Urinary GADD45G excretion is significantly associated with kidney disease progression in IgA nephropathy.
  • GADD45G is expressed in renal tubules of IgA nephropathy patients, suggesting a role in the disease process.
  • Urinary GADD45G may serve as a valuable non-invasive biomarker for monitoring IgA nephropathy progression.