Development of Hemispherical 3D Models of Human Brain and B Cell Lymphomas Using On-Chip Cell Dome System

Area of Science:

• Biotechnology
• Cancer Research
• Cell Biology

Background:

• Lymphocytes, crucial in lymphoma, are non-adherent, hindering the creation of realistic 3D tumor microenvironments.
• Existing 2D culture methods do not fully replicate the complex in vivo lymphoma milieu.

Purpose of the Study:

• To develop a novel method for fabricating 3D lymphoma models using an on-chip Cell Dome system.
• To investigate the behavior and drug response of lymphoma cells within these 3D structures.

Main Methods:

• Fabrication of hemispherical 3D structures using the on-chip Cell Dome system with a 1 mm diameter cavity.
• Culture of human brain lymphoma (TK) and B cell lymphoma (KML-1) cell lines within the 3D structures.
• Analysis of cell proliferation, hypoxia, CD19/CD20 expression, and doxorubicin resistance.

Main Results:

• TK and KML-1 cells proliferated and filled the hemispherical cavities, forming 3D structures with central hypoxic regions.
• CD20 expression showed differential regulation in TK and KML-1 cells within the 3D model compared to 2D cultures.
• Lymphoma cells in 3D Cell Dome cultures exhibited increased resistance to doxorubicin compared to 2D cultures.

Conclusions:

• The on-chip Cell Dome system is effective for fabricating functional 3D lymphoma models.
• These 3D models provide valuable insights into lymphoma microenvironment dynamics and drug response.
• The study supports the development of advanced 3D models for lymphoma research and therapeutic discovery.