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Fish Brain Cell Lines Can Be Infected with Adenoviral Vectors and Support Transgene Expression-An In Vitro Approach.

Alberto Cuesta1, Yulema Valero1

  • 1Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.

International Journal of Molecular Sciences
|January 8, 2025
PubMed
Summary
This summary is machine-generated.

Adenoviral vectors show potential for gene delivery in fish brain cells, aiding vaccine development against fish viruses. The Ad5/40 vector demonstrated improved infection and cellular distribution for targeted immune responses.

Keywords:
European sea bassadenoviral vectorbrainfishgilthead seabreamneurotropic virus

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Area of Science:

  • Aquatic animal health
  • Virology
  • Gene therapy

Background:

  • Designing effective vaccines for aquaculture fish viruses presents significant challenges.
  • Understanding host-pathogen interactions is crucial for developing novel fish disease control strategies.

Purpose of the Study:

  • To evaluate the potential of adenoviral (Ad) vectors, specifically Ad5 and chimeric Ad5/40, as gene delivery tools for fish brain cells.
  • To assess the infectivity and innate immune response of fish brain cell lines upon Ad vector infection.

Main Methods:

  • Infection of European sea bass (Dicentrarchus labrax) DLB-1 and gilthead seabream (Sparus aurata) SaB-1 brain cell lines with Ad5 and Ad5/40 vectors expressing GFP.
  • Evaluation of infection capacity using fluorescence microscopy and flow cytometry.
  • Assessment of antiviral innate immune response by quantifying the transcription of gene markers (irf3 and mx).

Main Results:

  • Both Ad5 and Ad5/40 vectors infected DLB-1 and SaB-1 cell lines, with Ad5/40 showing better infection and cellular distribution.
  • Infection efficiency was generally low, but Ad vector infection modulated the transcription of interferon-related antiviral immune response genes.
  • Fluorescence microscopy and flow cytometry confirmed vector presence in infected cells.

Conclusions:

  • Adenoviral vectors, particularly the Ad5/40 chimeric vector, show promise for targeted gene delivery to fish brain cells.
  • These vectors can induce an innate immune response, suggesting potential applications in developing localized antiviral strategies and vaccines for aquaculture fish.