Expanding the Fish-Brain Invitrome With the Senegalese Sole SsB-1 Cell Line-A Versatile Model for Neurotropic Virus...

Area of Science:

• * Aquaculture
• * Fish Virology
• * Cell Line Development

Background:

• * Established in vitro models are crucial for animal physiology and immunopathology research, aligning with the 3Rs principles.
• * The Senegalese sole (Solea senegalensis), important for Spanish aquaculture, currently lacks suitable in vitro models.
• * Developing cell lines supports sustainable aquaculture by providing alternatives to live animal studies.

Purpose of the Study:

• * To establish the first brain-derived cell line from Senegalese sole (Solea senegalensis).
• * To characterize the cell line for its potential use in studying virus-host interactions.
• * To provide a versatile platform for advancing virological and immunological research in marine aquaculture.

Main Methods:

• * Development and characterization of a novel brain-derived cell line from Senegalese sole.
• * Assessment of cell line morphology and glial features.
• * Infection studies using various fish viruses, including different genotypes of betanodavirus (NNV).

Main Results:

• * Successfully established SsB-1, the first brain-derived cell line from Senegalese sole.
• * SsB-1 cells exhibit stable glial features and morphology.
• * The cell line is highly susceptible to multiple betanodavirus genotypes, facilitating neurotropism and virulence studies, while showing limited susceptibility to IPNV, SVCV, and EVEX.

Conclusions:

• * SsB-1 provides a robust and ethical in vitro model for fish virology and immunology research.
• * The cell line's susceptibility profile aids in understanding virus-specific interactions and potential viral reservoir roles.
• * This development supports sustainable aquaculture by offering a valuable tool for disease research and prevention.